neurolucida 360 Search Results


spinal cord neurolucida 360  (MBF Bioscience)
90
MBF Bioscience spinal cord neurolucida 360
Spinal Cord Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/10__1523_slash_eneuro__0364___21__2021-99-12-17?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
spinal cord neurolucida 360 - by Bioz Stars, 2026-07
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built within neurolucida 360  (MBF Bioscience)
90
MBF Bioscience built within neurolucida 360
<t>Neurolucida</t> <t>360</t> tracing and digitizing of the TH-IR bundles and axons innervating the RA and LA (connected). ( a ) Tracing of TH-IR bundles revealed ~ 4 major bundles entered the atria mainly at the SVC, LPCV or LA-PV junction then bifurcated into small bundles that eventually ramified into individual varicose axons. Two bundles extended the TH-IR axons towards the RA while the other two bundles mainly innervated the LA. These four bundles innervated distinct regions with a certain degree of overlap. ( b ) TH-IR bundle that extended projections mainly towards the base of SVC, SAN, AVN, the lower part of the RAu. ( c ) A TH-IR bundle that extended projections towards the upper part of the RAu, SAN, LA and RA junction, and the medial part of the LA. ( d ) A TH-IR bundle that accessed the LA through the LPCV and mainly innervated the LPCV, the LAu, and the middle of the LA. ( e ) TH-IR bundles entered the LA between LPCV and the left PV to mainly innervate the LPCV, the left and middle PVs, the middle part of the LA and the junction of LA and RA. Arrows indicate the beginning of the parent axon bundle. RA right atrium, LA left atrium, LPCV left precaval vein, SVC superior vena cava. Scale bar: 500 μm.
Built Within Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc10082215-86-10-3?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
built within neurolucida 360 - by Bioz Stars, 2026-07
90/100 stars
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neurolucida 360 automated neuron reconstruction software  (MBF Bioscience)
90
MBF Bioscience neurolucida 360 automated neuron reconstruction software
(A) <t>Neurolucida</t> <t>360</t> showing inspection and correction of points (green) from the dendritic branch (yellow) that were drawn off center by the large spine (arrow). (B) To edit, view the branch in point mode, select the point, and move it to the correct, centered location on the dendritic branch. Scale bar = 0.5 µm.
Neurolucida 360 Automated Neuron Reconstruction Software, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc05113738-1055-14-20?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neurolucida 360 automated neuron reconstruction software - by Bioz Stars, 2026-07
90/100 stars
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neurolucida 360 dendritic tracing for sholl analysis  (MBF Bioscience)
90
MBF Bioscience neurolucida 360 dendritic tracing for sholl analysis
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Neurolucida 360 Dendritic Tracing For Sholl Analysis, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc07075964-265-12-7?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neurolucida 360 dendritic tracing for sholl analysis - by Bioz Stars, 2026-07
90/100 stars
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neurolucida 360 ver. 2021  (MBF Bioscience)
90
MBF Bioscience neurolucida 360 ver. 2021
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Neurolucida 360 Ver. 2021, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc10112881-100-0-3?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neurolucida 360 ver. 2021 - by Bioz Stars, 2026-07
90/100 stars
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automated neuronal tracing software neurolucida 360  (MBF Bioscience)
90
MBF Bioscience automated neuronal tracing software neurolucida 360
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Automated Neuronal Tracing Software Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pm31570162-106-15-20?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
automated neuronal tracing software neurolucida 360 - by Bioz Stars, 2026-07
90/100 stars
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computerbased cell tracing software neurolucida 360  (MBF Bioscience)
90
MBF Bioscience computerbased cell tracing software neurolucida 360
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Computerbased Cell Tracing Software Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/10__1523_slash_JNEUROSCI__0299___19__2019_ascii32_-151-1-7?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
computerbased cell tracing software neurolucida 360 - by Bioz Stars, 2026-07
90/100 stars
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neurolucida 360 dendritic tracing  (MBF Bioscience)
90
MBF Bioscience neurolucida 360 dendritic tracing
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Neurolucida 360 Dendritic Tracing, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc07075964__41467_2020_15224_MOESM1_ESM-147-9-7?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neurolucida 360 dendritic tracing - by Bioz Stars, 2026-07
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neurite detection with neurolucida 360  (MBF Bioscience)
90
MBF Bioscience neurite detection with neurolucida 360
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Neurite Detection With Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc11199630-143-21-23?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neurite detection with neurolucida 360 - by Bioz Stars, 2026-07
90/100 stars
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cell tracing software neurolucida 360  (MBF Bioscience)
90
MBF Bioscience cell tracing software neurolucida 360
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Cell Tracing Software Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pm38094594-150-0-6?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
cell tracing software neurolucida 360 - by Bioz Stars, 2026-07
90/100 stars
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neuron reconstruction module neurolucida 360  (MBF Bioscience)
90
MBF Bioscience neuron reconstruction module neurolucida 360
a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , <t>e</t> <t>Dendritic</t> complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, <t>Sholl</t> analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).
Neuron Reconstruction Module Neurolucida 360, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/pmc11735683-122-16-22?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neuron reconstruction module neurolucida 360 - by Bioz Stars, 2026-07
90/100 stars
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neurolucida 360® to generate a full, comprehensive, quantitative analysis of reconstructed structures  (MBF Bioscience)
90
MBF Bioscience neurolucida 360® to generate a full, comprehensive, quantitative analysis of reconstructed structures
Workflow for tracing the vasculature and CGRP-IR axon innervation of the vasculature in the stomach using Neurolucida 360®. (a) is a representative blood vessel that was innervated by CGRP-IR axons. (b) A closed contour was made around the perimeter of the blood vessel using Contour mode. The contour was converted to a flat surface to display the area of the stomach of which the blood vessel occupied. Here, only the arteries were contoured. (c) The blood vessel contour was temporarily hidden, the axons were traced using Neuron Tracing mode (please see Table 2 for a set of criteria of how to classify and trace the axons). (d) The contouring and tracing data were stacked to create a complete tracing image while the confocal image was removed. Scale bar = 100 µm. A higher resolution version of this figure is available in the Supporting Information.
Neurolucida 360® To Generate A Full, Comprehensive, Quantitative Analysis Of Reconstructed Structures, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurolucida+360/bio_rxiv__2023__05__23__541811-110-14-8?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
neurolucida 360® to generate a full, comprehensive, quantitative analysis of reconstructed structures - by Bioz Stars, 2026-07
90/100 stars
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Neurolucida 360 tracing and digitizing of the TH-IR bundles and axons innervating the RA and LA (connected). ( a ) Tracing of TH-IR bundles revealed ~ 4 major bundles entered the atria mainly at the SVC, LPCV or LA-PV junction then bifurcated into small bundles that eventually ramified into individual varicose axons. Two bundles extended the TH-IR axons towards the RA while the other two bundles mainly innervated the LA. These four bundles innervated distinct regions with a certain degree of overlap. ( b ) TH-IR bundle that extended projections mainly towards the base of SVC, SAN, AVN, the lower part of the RAu. ( c ) A TH-IR bundle that extended projections towards the upper part of the RAu, SAN, LA and RA junction, and the medial part of the LA. ( d ) A TH-IR bundle that accessed the LA through the LPCV and mainly innervated the LPCV, the LAu, and the middle of the LA. ( e ) TH-IR bundles entered the LA between LPCV and the left PV to mainly innervate the LPCV, the left and middle PVs, the middle part of the LA and the junction of LA and RA. Arrows indicate the beginning of the parent axon bundle. RA right atrium, LA left atrium, LPCV left precaval vein, SVC superior vena cava. Scale bar: 500 μm.

Journal: Scientific Reports

Article Title: Topographical mapping of catecholaminergic axon innervation in the flat-mounts of the mouse atria: a quantitative analysis

doi: 10.1038/s41598-023-27727-9

Figure Lengend Snippet: Neurolucida 360 tracing and digitizing of the TH-IR bundles and axons innervating the RA and LA (connected). ( a ) Tracing of TH-IR bundles revealed ~ 4 major bundles entered the atria mainly at the SVC, LPCV or LA-PV junction then bifurcated into small bundles that eventually ramified into individual varicose axons. Two bundles extended the TH-IR axons towards the RA while the other two bundles mainly innervated the LA. These four bundles innervated distinct regions with a certain degree of overlap. ( b ) TH-IR bundle that extended projections mainly towards the base of SVC, SAN, AVN, the lower part of the RAu. ( c ) A TH-IR bundle that extended projections towards the upper part of the RAu, SAN, LA and RA junction, and the medial part of the LA. ( d ) A TH-IR bundle that accessed the LA through the LPCV and mainly innervated the LPCV, the LAu, and the middle of the LA. ( e ) TH-IR bundles entered the LA between LPCV and the left PV to mainly innervate the LPCV, the left and middle PVs, the middle part of the LA and the junction of LA and RA. Arrows indicate the beginning of the parent axon bundle. RA right atrium, LA left atrium, LPCV left precaval vein, SVC superior vena cava. Scale bar: 500 μm.

Article Snippet: Additionally, Neurolucida Explorer (MBF Bioscience), an analytical software built within Neurolucida 360, was used to perform morphometric analysis on traced axon reconstructions.

Techniques:

(A) Neurolucida 360 showing inspection and correction of points (green) from the dendritic branch (yellow) that were drawn off center by the large spine (arrow). (B) To edit, view the branch in point mode, select the point, and move it to the correct, centered location on the dendritic branch. Scale bar = 0.5 µm.

Journal: Current protocols in neuroscience

Article Title: Automatic dendritic spine quantification from confocal data with Neurolucida 360

doi: 10.1002/cpns.16

Figure Lengend Snippet: (A) Neurolucida 360 showing inspection and correction of points (green) from the dendritic branch (yellow) that were drawn off center by the large spine (arrow). (B) To edit, view the branch in point mode, select the point, and move it to the correct, centered location on the dendritic branch. Scale bar = 0.5 µm.

Article Snippet: Zen Black image acquisition software (Zeiss) http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html AutoQuant image deconvolution software (Media Cybernetics) http://www.mediacy.com/index.aspx?page=AutoQuant Neurolucida 360 automated neuron reconstruction software (MBF Bioscience) http://www.mbfbioscience.com/neurolucida360 VIDEOS Automatic dendritic spine modeling with Neurolucida 360 ( DendriticSpines_with_Neurolucida360.mp4 ) This video demonstrates all steps in Neurolucida 360 for modeling dendritic spines on dendritic segments from loading the image data to classification.

Techniques:

A single image was created using Neurolucida 360 to montage multiple 3D images of a mouse hippocampal pyramidal neuron labeled with biocytin. Multiple 2-channel z-series images were collected with a Zeiss LSM 710 confocal microscope, mounted on an AxioImager Z2, with a 20× Plan-apochromat objective, and a 25 mW multi-wavelength (458/488/514) argon laser and a 20 mW 561 nm diode DPSS laser (Alexa-549 displayed here). Dr. Piskorowski provided the image data from an experiment performed by Vincent Robert and Ludivine Therreau in accordance with European guidelines for the care and use of laboratory animals at the Université Paris Descartes. Note: this neuron was not imaged at a resolution high enough for concurrent spine analysis. Scale bar = 100 µm.

Journal: Current protocols in neuroscience

Article Title: Automatic dendritic spine quantification from confocal data with Neurolucida 360

doi: 10.1002/cpns.16

Figure Lengend Snippet: A single image was created using Neurolucida 360 to montage multiple 3D images of a mouse hippocampal pyramidal neuron labeled with biocytin. Multiple 2-channel z-series images were collected with a Zeiss LSM 710 confocal microscope, mounted on an AxioImager Z2, with a 20× Plan-apochromat objective, and a 25 mW multi-wavelength (458/488/514) argon laser and a 20 mW 561 nm diode DPSS laser (Alexa-549 displayed here). Dr. Piskorowski provided the image data from an experiment performed by Vincent Robert and Ludivine Therreau in accordance with European guidelines for the care and use of laboratory animals at the Université Paris Descartes. Note: this neuron was not imaged at a resolution high enough for concurrent spine analysis. Scale bar = 100 µm.

Article Snippet: Zen Black image acquisition software (Zeiss) http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html AutoQuant image deconvolution software (Media Cybernetics) http://www.mediacy.com/index.aspx?page=AutoQuant Neurolucida 360 automated neuron reconstruction software (MBF Bioscience) http://www.mbfbioscience.com/neurolucida360 VIDEOS Automatic dendritic spine modeling with Neurolucida 360 ( DendriticSpines_with_Neurolucida360.mp4 ) This video demonstrates all steps in Neurolucida 360 for modeling dendritic spines on dendritic segments from loading the image data to classification.

Techniques: Labeling, Microscopy

Using Neurolucida 360, the pyramidal cell shown in Figure 11 was reconstructed using user-guided tracing and soma modeling. Scale bar = 100 µm.

Journal: Current protocols in neuroscience

Article Title: Automatic dendritic spine quantification from confocal data with Neurolucida 360

doi: 10.1002/cpns.16

Figure Lengend Snippet: Using Neurolucida 360, the pyramidal cell shown in Figure 11 was reconstructed using user-guided tracing and soma modeling. Scale bar = 100 µm.

Article Snippet: Zen Black image acquisition software (Zeiss) http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html AutoQuant image deconvolution software (Media Cybernetics) http://www.mediacy.com/index.aspx?page=AutoQuant Neurolucida 360 automated neuron reconstruction software (MBF Bioscience) http://www.mbfbioscience.com/neurolucida360 VIDEOS Automatic dendritic spine modeling with Neurolucida 360 ( DendriticSpines_with_Neurolucida360.mp4 ) This video demonstrates all steps in Neurolucida 360 for modeling dendritic spines on dendritic segments from loading the image data to classification.

Techniques:

Troubleshooting

Journal: Current protocols in neuroscience

Article Title: Automatic dendritic spine quantification from confocal data with Neurolucida 360

doi: 10.1002/cpns.16

Figure Lengend Snippet: Troubleshooting

Article Snippet: Zen Black image acquisition software (Zeiss) http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html AutoQuant image deconvolution software (Media Cybernetics) http://www.mediacy.com/index.aspx?page=AutoQuant Neurolucida 360 automated neuron reconstruction software (MBF Bioscience) http://www.mbfbioscience.com/neurolucida360 VIDEOS Automatic dendritic spine modeling with Neurolucida 360 ( DendriticSpines_with_Neurolucida360.mp4 ) This video demonstrates all steps in Neurolucida 360 for modeling dendritic spines on dendritic segments from loading the image data to classification.

Techniques: Labeling, Microscopy, Imaging, Software, Expressing

a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , e Dendritic complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, Sholl analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).

Journal: Nature Communications

Article Title: Lupus autoantibodies act as positive allosteric modulators at GluN2A-containing NMDA receptors and impair spatial memory

doi: 10.1038/s41467-020-15224-w

Figure Lengend Snippet: a Schematic of NMDAR composition expected in hippocampal pyramidal neurons in the various genetic backgrounds (Supplementary Fig. 2). b Micrographs of hippocampal CA1 neurons from DNRAb+ or DNRAb− N2B cKO or N2A KO mice. Scale bar, 20 µm. c Quantification of CA1 neurons (mean ± SEM, n = 24 sections per group). Each dot represents a CA1 pyramidal neuron field counted using a standard unbiased stereological protocol (eight runs of systematic random sampling in three animals per group) ( ***p < 0.001, two-sided Mann–Whitney U test) (left to right, p = 0.0000156, 0.765). d , e Dendritic complexity of Golgi-stained neurons. Left, tracings of CA1 pyramidal neurons from N2B cKO ( d ) or N2A KO ( e ) mice. Right, Sholl analysis for N2B cKO ( d ) or N2A KO ( e ) mice (*** p < 0.001, two-sided Kolmogorov–Smirnov test for data converted to cumulative distribution function) (left to right, p = 2.754 × 10 −16 , 0.61). Values at concentric rings are shown as mean ± SEM. For each group, 10–20 neurons per animal, 4 animals per group. f , g Representative images of microglia from N2B cKO ( a ) or N2A KO ( b ) mice. Microglia are labeled with antibody to Iba1 (red, Alexa 594) and to CD68 (green, Alexa-488). Scale (white bar): 5 µm. Right panels, quantification of CD68 + score (mean ± SEM) (*** p < 0.001, Kruskal–Wallis ANOVA test) (left to right, p = 0.00000000222, 0.0793) (see Supplementary Methods). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 42), DNRAb+ ( n = 87), N2A KO, DNRAb− ( n = 57), DNRAb+ ( n = 58). h , i Microglia process complexity and length. Left panels, Sholl analysis of process length of microglia from N2B cKO ( h ) or N2A KO ( i ) mice. Right panels, cumulative probability distribution curves of microglia process length from N2B cKO ( h ) or N2A KO ( i ) (*** p < 0.001, two-sided Kolmogorov–Smirnov test) (top to bottom, p = 7.16 × 10 −8 , 0.999). Three animals per group; number of quantified microglia: N2B cKO, DNRAb− ( n = 33), DNRAb+ ( n = 34); N2A KO, DNRAb− ( n = 39), DNRAb+ ( n = 40).

Article Snippet: Images were then analyzed using Neurolucida 360 (MBF Bioscience) dendritic tracing for Sholl analysis at 10 μm shells from the soma , .

Techniques: Sampling, MANN-WHITNEY, Staining, Labeling

Workflow for tracing the vasculature and CGRP-IR axon innervation of the vasculature in the stomach using Neurolucida 360®. (a) is a representative blood vessel that was innervated by CGRP-IR axons. (b) A closed contour was made around the perimeter of the blood vessel using Contour mode. The contour was converted to a flat surface to display the area of the stomach of which the blood vessel occupied. Here, only the arteries were contoured. (c) The blood vessel contour was temporarily hidden, the axons were traced using Neuron Tracing mode (please see Table 2 for a set of criteria of how to classify and trace the axons). (d) The contouring and tracing data were stacked to create a complete tracing image while the confocal image was removed. Scale bar = 100 µm. A higher resolution version of this figure is available in the Supporting Information.

Journal: bioRxiv

Article Title: Mapping the Organization and Morphology of Calcitonin Gene-Related Peptide (CGRP)-IR Axons in the Whole Mouse Stomach

doi: 10.1101/2023.05.23.541811

Figure Lengend Snippet: Workflow for tracing the vasculature and CGRP-IR axon innervation of the vasculature in the stomach using Neurolucida 360®. (a) is a representative blood vessel that was innervated by CGRP-IR axons. (b) A closed contour was made around the perimeter of the blood vessel using Contour mode. The contour was converted to a flat surface to display the area of the stomach of which the blood vessel occupied. Here, only the arteries were contoured. (c) The blood vessel contour was temporarily hidden, the axons were traced using Neuron Tracing mode (please see Table 2 for a set of criteria of how to classify and trace the axons). (d) The contouring and tracing data were stacked to create a complete tracing image while the confocal image was removed. Scale bar = 100 µm. A higher resolution version of this figure is available in the Supporting Information.

Article Snippet: Another software by MBF Biosciences, Neurolucida Explorer® ( https://www.mbfbioscience.com/neurolucida-explorer , RRID:SCR_017348), goes hand-in-hand with Neurolucida 360® to generate a full, comprehensive, quantitative analysis of reconstructed structures.

Techniques:

Reconstructed CGRP-IR axons of a ventral mouse stomach using Neurolucida 360. (a) Digitization of the vasculature displays the blood vessels’ distinctive distribution pattern. (b) CGRP-IR axons innervating the vasculature in stomach (a) as well as those in the muscular and myenteric plexus layers were identified and traced. These axons were assigned with a random color by the software. (c, d) Ventral stomachs of other animals also shared similar CGRP-IR axon innervation patterns. Scale bar = 1000 µm. A higher resolution version of this figure is available in the Supporting Information.

Journal: bioRxiv

Article Title: Mapping the Organization and Morphology of Calcitonin Gene-Related Peptide (CGRP)-IR Axons in the Whole Mouse Stomach

doi: 10.1101/2023.05.23.541811

Figure Lengend Snippet: Reconstructed CGRP-IR axons of a ventral mouse stomach using Neurolucida 360. (a) Digitization of the vasculature displays the blood vessels’ distinctive distribution pattern. (b) CGRP-IR axons innervating the vasculature in stomach (a) as well as those in the muscular and myenteric plexus layers were identified and traced. These axons were assigned with a random color by the software. (c, d) Ventral stomachs of other animals also shared similar CGRP-IR axon innervation patterns. Scale bar = 1000 µm. A higher resolution version of this figure is available in the Supporting Information.

Article Snippet: Another software by MBF Biosciences, Neurolucida Explorer® ( https://www.mbfbioscience.com/neurolucida-explorer , RRID:SCR_017348), goes hand-in-hand with Neurolucida 360® to generate a full, comprehensive, quantitative analysis of reconstructed structures.

Techniques: Software

Reconstructed CGRP-IR axons of a dorsal mouse stomach using Neurolucida 360. (a) Digitization of the vasculature displays the blood vessels’ distinctive distribution pattern. (b) CGRP-IR axons innervating the vasculature in stomach (a) as well as those in the muscular and myenteric plexus layers were identified and traced. These axons were assigned with a random color by the software. (c, d) Dorsal stomachs of other animals also shared similar CGRP-IR axon innervation patterns. Scale bar = 1000 µm. A higher resolution version of this figure is available in the Supporting Information.

Journal: bioRxiv

Article Title: Mapping the Organization and Morphology of Calcitonin Gene-Related Peptide (CGRP)-IR Axons in the Whole Mouse Stomach

doi: 10.1101/2023.05.23.541811

Figure Lengend Snippet: Reconstructed CGRP-IR axons of a dorsal mouse stomach using Neurolucida 360. (a) Digitization of the vasculature displays the blood vessels’ distinctive distribution pattern. (b) CGRP-IR axons innervating the vasculature in stomach (a) as well as those in the muscular and myenteric plexus layers were identified and traced. These axons were assigned with a random color by the software. (c, d) Dorsal stomachs of other animals also shared similar CGRP-IR axon innervation patterns. Scale bar = 1000 µm. A higher resolution version of this figure is available in the Supporting Information.

Article Snippet: Another software by MBF Biosciences, Neurolucida Explorer® ( https://www.mbfbioscience.com/neurolucida-explorer , RRID:SCR_017348), goes hand-in-hand with Neurolucida 360® to generate a full, comprehensive, quantitative analysis of reconstructed structures.

Techniques: Software